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While in practice we've used it with pixel sizes ranging from 9.2Å to 25.0Å, it is probably ideal to run it at a pixel size close to 10Å. For that you may need to downscale your tomogram. You can do that by fourier shrink your tomogram with EMAN2. Lets say you have a Tomogram with a pixelsize of 5.9359Å. The fouriershrink factor is then 10Å/5.9359Å = 1.684 +TomoTwin has been trained on tomograms with a pixel size of 10Å. While in practice we've used it with pixel sizes ranging from 9.2Å to 25.0Å, it's probably often ideal to run it with a pixel size close to 10Å. However, for proteins equal to or larger than the ribosome, we have found that a larger pixel size (e.g. 15Å) works better. For this you may need to rescale your tomogram. You can do this by Fourier shrinking your tomogram with EMAN2. Suppose you have a tomogram with a pixel size of 5.9359Å. The Fourier shrink factor is then 10Å/5.9359Å = 1.684 diff --git a/docs/tutorials/tut01_reference.rst b/docs/tutorials/tut01_reference.rst index c9d668b..2350713 100644 --- a/docs/tutorials/tut01_reference.rst +++ b/docs/tutorials/tut01_reference.rst @@ -14,7 +14,7 @@ In this tutorial we describe how to use TomoTwin for picking in tomograms using Download: `https `_ -1. Downscale your Tomogram to 10 Å +1. Rescale your Tomogram ---------------------------------- .. include:: text_modules/downscale_reference.rst diff --git a/docs/tutorials/tut02_cluster.rst b/docs/tutorials/tut02_cluster.rst index 18fbe4e..4aede12 100644 --- a/docs/tutorials/tut02_cluster.rst +++ b/docs/tutorials/tut02_cluster.rst @@ -4,7 +4,7 @@ Tutorial 2: Clustering based particle picking -1. Downscale your Tomogram to 10 Å +1. Rescale your Tomogram --------------------------------- .. include:: text_modules/downscale_clustering.rst