diff --git a/DESCRIPTION b/DESCRIPTION index 70320cf..6d8a030 100644 --- a/DESCRIPTION +++ b/DESCRIPTION @@ -1,6 +1,6 @@ Package: MACSr Title: MACS: Model-based Analysis for ChIP-Seq -Version: 1.11.3 +Version: 1.13.1 Authors@R: c( person(given = "Philippa", family = "Doherty", diff --git a/NEWS b/NEWS index 8c3dd51..bb8f9d4 100644 --- a/NEWS +++ b/NEWS @@ -1,4 +1,3 @@ -Changes in version 1.11.3 (2024-9-12) +Changes in version 1.13.1 (2024-9-19) + Upgrade to MACS 3.0.2. -Changes in version 1.11.2 (2023-11-20) -+ Upgrade to MACS 3.0.0. + diff --git a/inst/extdata/CTCF_PE_CTRL_chr22_50k.bam b/inst/extdata/CTCF_PE_CTRL_chr22_50k.bam deleted file mode 100644 index 7994585..0000000 Binary files a/inst/extdata/CTCF_PE_CTRL_chr22_50k.bam and /dev/null differ diff --git a/inst/extdata/CTCF_PE_ChIP_chr22_50k.bam b/inst/extdata/CTCF_PE_ChIP_chr22_50k.bam deleted file mode 100644 index 2fd9469..0000000 Binary files a/inst/extdata/CTCF_PE_ChIP_chr22_50k.bam and /dev/null differ diff --git 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0.30200 -chr22 51217313 51217541 0.94612 -chr22 51217541 51217552 0.30200 -chr22 51217552 51217569 0.28043 -chr22 51217569 51218048 0.25886 -chr22 51218048 51218398 0.23729 -chr22 51218398 51218760 0.21572 -chr22 51218760 51218988 0.94612 -chr22 51218988 51219269 0.23729 -chr22 51219269 51219324 0.25886 -chr22 51219324 51219374 0.43143 -chr22 51219374 51219710 0.25886 -chr22 51219710 51219938 0.94612 -chr22 51219938 51220080 0.25886 -chr22 51220080 51220324 0.43143 -chr22 51220324 51220466 0.25886 -chr22 51220466 51220694 0.94612 -chr22 51220694 51221284 0.25886 -chr22 51221284 51221518 0.43143 -chr22 51221518 51221670 0.94612 -chr22 51221670 51221746 1.89225 -chr22 51221746 51222040 1.07858 -chr22 51222040 51222114 1.89225 -chr22 51222114 51222134 2.83837 -chr22 51222134 51222268 1.89225 -chr22 51222268 51222342 0.94612 -chr22 51222342 51222520 0.64715 -chr22 51222520 51222548 0.43143 diff --git a/inst/extdata/yeast_500k_SRR1822137.bam b/inst/extdata/yeast_500k_SRR1822137.bam deleted file mode 100644 index 009c9fe..0000000 Binary files a/inst/extdata/yeast_500k_SRR1822137.bam and /dev/null differ diff --git a/inst/extdata/yeast_500k_SRR1822137.bedpe.gz b/inst/extdata/yeast_500k_SRR1822137.bedpe.gz deleted file mode 100644 index ad10819..0000000 Binary files a/inst/extdata/yeast_500k_SRR1822137.bedpe.gz and /dev/null differ diff --git a/tests/testthat/test-init.R b/tests/testthat/test-init.R index 9516969..c90bf6c 100644 --- a/tests/testthat/test-init.R +++ b/tests/testthat/test-init.R @@ -1,13 +1,13 @@ -# datdir <- system.file("extdata", package = "MACSr") -# eh <- ExperimentHub::ExperimentHub() -# eh <- AnnotationHub::query(eh, "MACSdata") -# CHIP <- eh[["EH4558"]] -# CTRL <- eh[["EH4563"]] -# CHIPPE <- eh[["EH4559"]] -# CTRLPE <- eh[["EH4564"]] -# CHIPBEDPE <- eh[["EH4560"]] -# CTRLBEDPE <- eh[["EH4565"]] +datdir <- system.file("extdata", package = "MACSr") +eh <- ExperimentHub::ExperimentHub() +eh <- AnnotationHub::query(eh, "MACSdata") +CHIP <- eh[["EH4558"]] +CTRL <- eh[["EH4563"]] +CHIPPE <- eh[["EH4559"]] +CTRLPE <- eh[["EH4564"]] +CHIPBEDPE <- eh[["EH4560"]] +CTRLBEDPE <- eh[["EH4565"]] # context("1. test callpeak") @@ -113,16 +113,16 @@ ## atac <- hmmratac(bam=ATACSEQBAM, outdir="/tmp/atac", name="hmmratac_yeast500k", save_train=TRUE) -datdir <- system.file("extdata", package = "MACSr") +# datdir <- system.file("extdata", package = "MACSr") -CHIP <- file.path(datdir, "CTCF_SE_ChIP_chr22_50k.bed.gz") -CTRL <- file.path(datdir, "CTCF_SE_CTRL_chr22_50k.bed.gz") +# CHIP <- file.path(datdir, "CTCF_SE_ChIP_chr22_50k.bed.gz") +# CTRL <- file.path(datdir, "CTCF_SE_CTRL_chr22_50k.bed.gz") -CHIPPE <- file.path(datdir, "CTCF_PE_ChIP_chr22_50k.bam") -CTRLPE <- file.path(datdir, "CTCF_PE_CTRL_chr22_50k.bam") +# CHIPPE <- file.path(datdir, "CTCF_PE_ChIP_chr22_50k.bam") +# CTRLPE <- file.path(datdir, "CTCF_PE_CTRL_chr22_50k.bam") -CHIPBEDPE <- file.path(datdir, "CTCF_PE_ChIP_chr22_50k.bedpe.gz") -CTRLBEDPE <- file.path(datdir, "CTCF_PE_CTRL_chr22_50k.bedpe.gz") +# CHIPBEDPE <- file.path(datdir, "CTCF_PE_ChIP_chr22_50k.bedpe.gz") +# CTRLBEDPE <- file.path(datdir, "CTCF_PE_CTRL_chr22_50k.bedpe.gz") context("1. test callpeak") @@ -173,19 +173,19 @@ context("5. test randsample") outputfile = "randsample_output.bed")) }) -context("6. test refinepeak") - test_that("refinepeak runs without error 1", { - expect_no_error(rp1 <- refinepeak( - bedfile = file.path(datdir, 'run_callpeak_narrow0_peaks.narrowPeak'), - ifile = CHIP, - oprefix="refinepeak_output_prefix")) - }) - test_that("refinepeak runs without error 2", { - expect_no_error(rp2 <- refinepeak( - bedfile = file.path(datdir, 'run_callpeak_narrow0_peaks.narrowPeak'), - ifile = CHIP, - outputfile="refinepeak_output.bed")) - }) +# context("6. test refinepeak") +# test_that("refinepeak runs without error 1", { +# expect_no_error(rp1 <- refinepeak( +# bedfile = file.path(datdir, 'run_callpeak_narrow0_peaks.narrowPeak'), +# ifile = CHIP, +# oprefix="refinepeak_output_prefix")) +# }) +# test_that("refinepeak runs without error 2", { +# expect_no_error(rp2 <- refinepeak( +# bedfile = file.path(datdir, 'run_callpeak_narrow0_peaks.narrowPeak'), +# ifile = CHIP, +# outputfile="refinepeak_output.bed")) +# }) context("7. test bdgcmp") test_that("bdgcmp runs without error 1", { @@ -205,63 +205,63 @@ context("7. test bdgcmp") outputfile= list("bdgcmp_output.bdg") )) # why is it defined as a list? }) -context("8. test bdgpeakcall") - test_that("bdgpeakcall runs without error 1", { - expect_no_error(bdgpc1 <- bdgpeakcall( - ifile = file.path(datdir, 'run_bdgcmp_FE.bdg'), - cutoff = 2, - oprefix="bdgpeakcall_output_prefix")) - }) - test_that("bdgpeakcall runs without error 2", { - expect_no_error(bdgpc2 <- bdgpeakcall( - ifile = file.path(datdir, 'run_bdgcmp_FE.bdg'), - cutoff = 2, - outputfile="bdgpeakcall_output.txt")) - }) - -context("9. test bdgbroadcall") - test_that("bdgbroadcall runs without error 1", { - expect_no_error(bdgbc1 <- bdgbroadcall( - ifile = file.path(datdir, 'run_bdgcmp_FE.bdg'), - cutoffpeak=2, - cutofflink=1.5, - oprefix="bdgbroadcall_output" )) - }) - test_that("bdgbroadcall runs without error 2", { - expect_no_error(bdgbc2 <- bdgbroadcall( - ifile = file.path(datdir, 'run_bdgcmp_FE.bdg'), - cutoffpeak=2, - cutofflink=1.5, - outputfile="bdgbroadcall_output.bed" )) - }) - -context("10. test bdgdiff") - test_that("bdgdiff runs without error 1", { - expect_no_error(bdgdiff1 <- bdgdiff( - t1bdg = file.path(datdir, 'run_callpeak_narrow0_treat_pileup.bdg'), - c1bdg = file.path(datdir, 'run_callpeak_narrow0_control_lambda.bdg'), - t2bdg = file.path(datdir, 'run_callpeak_narrow_revert_treat_pileup.bdg'), - c2bdg = file.path(datdir, 'run_callpeak_narrow_revert_control_lambda.bdg'), - oprefix="bdgdiff_output_prefix")) - }) - test_that("bdgdiff runs without error 2", { - expect_no_error(bdgdiff2 <- bdgdiff( - t1bdg = file.path(datdir, 'run_callpeak_narrow0_treat_pileup.bdg'), - c1bdg = file.path(datdir, 'run_callpeak_narrow0_control_lambda.bdg'), - t2bdg = file.path(datdir, 'run_callpeak_narrow_revert_treat_pileup.bdg'), - c2bdg = file.path(datdir, 'run_callpeak_narrow_revert_control_lambda.bdg'), - outputfile= list("bdgdiff_output1.bed", "bdgdiff_output2.bed", "bdgdiff_output3.bed"))) - }) - -context("11. test cmbreps") - test_that("cmbreps runs without error", { - expect_no_error(cmbreps <- cmbreps( - ifile = list(file.path(datdir, 'run_callpeak_narrow0_treat_pileup.bdg'), - file.path(datdir, 'run_callpeak_narrow0_control_lambda.bdg')), - method = "max", - outputfile = "cmbreps_output.bdg" - )) - }) +# context("8. test bdgpeakcall") +# test_that("bdgpeakcall runs without error 1", { +# expect_no_error(bdgpc1 <- bdgpeakcall( +# ifile = file.path(datdir, 'run_bdgcmp_FE.bdg'), +# cutoff = 2, +# oprefix="bdgpeakcall_output_prefix")) +# }) +# test_that("bdgpeakcall runs without error 2", { +# expect_no_error(bdgpc2 <- bdgpeakcall( +# ifile = file.path(datdir, 'run_bdgcmp_FE.bdg'), +# cutoff = 2, +# outputfile="bdgpeakcall_output.txt")) +# }) + +# context("9. test bdgbroadcall") +# test_that("bdgbroadcall runs without error 1", { +# expect_no_error(bdgbc1 <- bdgbroadcall( +# ifile = file.path(datdir, 'run_bdgcmp_FE.bdg'), +# cutoffpeak=2, +# cutofflink=1.5, +# oprefix="bdgbroadcall_output" )) +# }) +# test_that("bdgbroadcall runs without error 2", { +# expect_no_error(bdgbc2 <- bdgbroadcall( +# ifile = file.path(datdir, 'run_bdgcmp_FE.bdg'), +# cutoffpeak=2, +# cutofflink=1.5, +# outputfile="bdgbroadcall_output.bed" )) +# }) + +# context("10. test bdgdiff") +# test_that("bdgdiff runs without error 1", { +# expect_no_error(bdgdiff1 <- bdgdiff( +# t1bdg = file.path(datdir, 'run_callpeak_narrow0_treat_pileup.bdg'), +# c1bdg = file.path(datdir, 'run_callpeak_narrow0_control_lambda.bdg'), +# t2bdg = file.path(datdir, 'run_callpeak_narrow_revert_treat_pileup.bdg'), +# c2bdg = file.path(datdir, 'run_callpeak_narrow_revert_control_lambda.bdg'), +# oprefix="bdgdiff_output_prefix")) +# }) +# test_that("bdgdiff runs without error 2", { +# expect_no_error(bdgdiff2 <- bdgdiff( +# t1bdg = file.path(datdir, 'run_callpeak_narrow0_treat_pileup.bdg'), +# c1bdg = file.path(datdir, 'run_callpeak_narrow0_control_lambda.bdg'), +# t2bdg = file.path(datdir, 'run_callpeak_narrow_revert_treat_pileup.bdg'), +# c2bdg = file.path(datdir, 'run_callpeak_narrow_revert_control_lambda.bdg'), +# outputfile= list("bdgdiff_output1.bed", "bdgdiff_output2.bed", "bdgdiff_output3.bed"))) +# }) + +# context("11. test cmbreps") +# test_that("cmbreps runs without error", { +# expect_no_error(cmbreps <- cmbreps( +# ifile = list(file.path(datdir, 'run_callpeak_narrow0_treat_pileup.bdg'), +# file.path(datdir, 'run_callpeak_narrow0_control_lambda.bdg')), +# method = "max", +# outputfile = "cmbreps_output.bdg" +# )) +# }) context("12. test bdgopt") test_that("bdgopt runs without error", { @@ -272,29 +272,29 @@ context("12. test bdgopt") outputfile="bdgopt_output.bdg" )) }) -context("13. test callvar") - test_that("callvar runs without error", { - expect_no_error(cv1 <- callvar( - peakbed=file.path(datdir, "callvar_testing.narrowPeak"), - tfile=CHIPPE, - cfile=CTRLPE, - outputfile="callvar_output.vcf" - )) - }) +# context("13. test callvar") +# test_that("callvar runs without error", { +# expect_no_error(cv1 <- callvar( +# peakbed=file.path(datdir, "callvar_testing.narrowPeak"), +# tfile=CHIPPE, +# cfile=CTRLPE, +# outputfile="callvar_output.vcf" +# )) +# }) # context("14. test 50k contigs with buffersize") -context("15. test hmmratac") - test_that("hmmratac runs without error, defaults", { - expect_no_error(hm1 <- hmmratac( - input_file = file.path(datdir, 'yeast_500k_SRR1822137.bam'), - name = "hmmratac_output")) - }) - test_that("hmmratac runs without error, format = BEDPE", { - expect_no_error(hm2 <- hmmratac( - input_file = file.path(datdir, 'yeast_500k_SRR1822137.bedpe.gz'), - format = 'BEDPE', - name = "hmmratac_bedpe_output")) - }) +# context("15. test hmmratac") +# test_that("hmmratac runs without error, defaults", { +# expect_no_error(hm1 <- hmmratac( +# input_file = file.path(datdir, 'yeast_500k_SRR1822137.bam'), +# name = "hmmratac_output")) +# }) +# test_that("hmmratac runs without error, format = BEDPE", { +# expect_no_error(hm2 <- hmmratac( +# input_file = file.path(datdir, 'yeast_500k_SRR1822137.bedpe.gz'), +# format = 'BEDPE', +# name = "hmmratac_bedpe_output")) +# })