diff --git a/docs/README.md b/docs/README.md index 9fcafa16..63bb918f 100644 --- a/docs/README.md +++ b/docs/README.md @@ -1,5 +1,7 @@ # Welcome to `recOrder`'s documentation +**I want to buy hardware for a polarized-light installation:** start with the [buyers guide](./buyers-guide.md). + **I would like to install `recOrder` on my microscope:** start with the [microscope installation guide](./microscope-installation-guide.md). **I would like to use the `napari plugin`:** start with the [plugin guide](./napari-plugin-guide.md). diff --git a/docs/buyers-guide.md b/docs/buyers-guide.md new file mode 100644 index 00000000..6b64a7b7 --- /dev/null +++ b/docs/buyers-guide.md @@ -0,0 +1,39 @@ +# Buyer's Guide + +## Quantitative phase imaging: + +You can use a transmitted light source (LED or a lamp) and a condenser commonly available on almost all microscopes. In addition to the transmitted light imaging path, you will need a motorized stage for acquiring through-focus image stacks. + +## Quantitative polarization imaging (PolScope): + +The following list of components assumes that you already have a transmitted light source (LED or a lamp) and a condenser. + +Buyers have two options: +1. buy a complete hardware kit from the OpenPolScope project, or +2. assemble your own kit piece by piece. + +### Buy a kit from the OpenPolScope project + +- Read about the [OpenPolScope Hardware Kit](https://openpolscope.org/pages/OPS_Hardware.htm). +- Complete the [OpenPolScope information request form](https://openpolscope.org/pages/Info_Request_Form.htm). + +### Buy individual components + +The components are listed in the order in which they process light. See the build video here to see how to assemble these components on your microscope. + +https://github.com/user-attachments/assets/a0a8bffb-bf81-4401-9ace-3b4955436b57 + +| Part | Approximate Price | Notes | +|--------------------------|-------------------|-----------------------------| +| Illumination filter | $200 | We suggest [a Thorlabs CWL = 530 nm, FWHM = 10 nm notch filter](https://www.thorlabs.com/thorproduct.cfm?partnumber=FBH530-10).| +| Circular polarizer | $350 | We suggest [a Thorlabs 532 nm, left-hand circular polarizer](https://www.thorlabs.com/thorproduct.cfm?partnumber=CP1L532).| +| Liquid crystal compensator | $6,000 | Meadowlark optics LVR-42x52mm-VIS-ASSY or LVR-50x60mm-VIS-POL-ASSY. Although near-variants are listed in the [Meadowlowlark catalog](https://www.meadowlark.com/product/liquid-crystal-variable-retarder/), this is a custom part with two liquid crystals in a custom housing. [Contact Meadowlark](https://www.meadowlark.com/contact-us/) for a quote.| +| Liquid crystal control electronics | $2,000 | [Meadowlark optics D5020-20V](https://www.meadowlark.com/product/liquid-crystal-digital-interface-controller/). Choose the high-voltage 20V version. +| Liquid crystal adapter | $25-$500 | A 3D printed part that aligns the liquid crystal compensator in a microscope stand's illumination path. Check for your stand among the [OpenPolScope `.stl` files](https://github.com/amitabhverma/Microscope-LC-adapters/tree/main/stl_files) or [contact us](compmicro@czbiohub.org) for more options.| +| Circular analyzer (opposite handedness) | $350 | We suggest [a Thorlabs 532 nm, right-hand circular polarizer](https://www.thorlabs.com/thorproduct.cfm?partnumber=CP1R532).| + +If you need help selecting or assembling the components, please start an issue on this GitHub repository or contact us at compmicro@czbiohub.org. + +## Quantitative phase and polarization imaging (QLIPP): + +Combining the Z-stage and the PolScope components listed above enables joint phase and polarization imaging with `recOrder`. \ No newline at end of file