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number of contigs increased after falcon-phase #81

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olechnwin opened this issue May 7, 2021 · 0 comments
Open

number of contigs increased after falcon-phase #81

olechnwin opened this issue May 7, 2021 · 0 comments

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@olechnwin
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Hello,

I tried running FALCON-Phase via pb-assembly. I got the following assembly stats after falcon-unzip:

python ~/opt/pb-assembly/scripts/get_asm_stats.py 4-polish/cns-output/polished_p_ctgs.fasta
	{
	 "asm_contigs": 3025,
	 "asm_esize": 7985624,
	 "asm_max": 37598334,
	 "asm_mean": 967830,
	 "asm_median": 102824,
	 "asm_min": 11443,
	 "asm_n50": 5909326,
	 "asm_n90": 632505,
	 "asm_n95": 249651,
	 "asm_total_bp": 2927686349
	}

python ~/opt/pb-assembly/scripts/get_asm_stats.py 4-polish/cns-output/polished_h_ctgs.fasta
	{
	 "asm_contigs": 26845,
	 "asm_esize": 87309,
	 "asm_max": 797903,
	 "asm_mean": 48371,
	 "asm_median": 34312,
	 "asm_min": 9716,
	 "asm_n50": 61500,
	 "asm_n90": 24083,
	 "asm_n95": 19645,
	 "asm_total_bp": 1298537655
	}

I ran FALCON-phase with the following configuration:

[Phase]
	cns_p_ctg_fasta = Data/A673_pacbio/HiFi/falcon_hifi/4-polish/cns-output/polished_p_ctgs.fasta
	cns_h_ctg_fasta = Data/A673_pacbio/HiFi/falcon_hifi/4-polish/cns-output/polished_h_ctgs.fasta
	reads_1=Data/A673_KD_Rescue_HiC/A673_Parental_Rep1_R1.fastq.gz
	reads_2=Data/A673_KD_Rescue_HiC/A673_Parental_Rep1_R2.fastq.gz
	min_aln_len=3000
	iterations=10000000
	enzyme="GATC,GAATC,GACTC,GATTC,GAGTC"
	output_format=pseudohap

and got the following assembly result:

python ~/opt/pb-assembly/scripts/get_asm_stats.py 5-phase/output/phased.0.fasta
	{
	 "asm_contigs": 54347,
	 "asm_esize": 714472,
	 "asm_max": 15330875,
	 "asm_mean": 53884,
	 "asm_median": 27435,
	 "asm_min": 1,
	 "asm_n50": 99079,
	 "asm_n90": 22607,
	 "asm_n95": 15846,
	 "asm_total_bp": 2928459876
	}

which shows the number of contigs increases from 3025 to 54347. Is it supposed to be like this? I was expecting the number of contigs to go down. Although I also see the n50 increases.
Can someone please advise. Thank you in advance!

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