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I'm experiencing an issue when using CytoNorm.normalize(). The function properly generates the new normalized fcs files, and they are placed in the folder I specify in outputDir. However, there are also a bunch of other .fcs files created whose filenames appear to be the filepaths to the input data.
Here is an example of the files I find in the specified outputDir:
Correctly named sample example (there are 8 files, as expected):
Norm_BAS_SUR_AA22008_2022-03-14_Peanut1_QC.fcs
Unknown files example names (there are 100 of these files):
C__Users_emili_Desktop_emilier_flowSOM_results_QC_PeacoQC_results_fcs_files_BAS_SUR_AA23013_2024-07-31_Peanut_QC.fcs_fsom1.fcs
What is the potential cause for these unknown files to be created and not removed ? What should I change to the script to fix this issue?
Thank you!
The text was updated successfully, but these errors were encountered:
emilier17
changed the title
CytoNorm.normalize() creating
CytoNorm.normalize() creating unknown .fcs files
Feb 25, 2025
Hello,
I'm experiencing an issue when using CytoNorm.normalize(). The function properly generates the new normalized fcs files, and they are placed in the folder I specify in outputDir. However, there are also a bunch of other .fcs files created whose filenames appear to be the filepaths to the input data.
Here is how I used the function:
CytoNorm.normalize(model = norm_model,
files = sample_files,
labels = sample_labels,
transformList = NULL,
transformList.reverse = NULL,
normMethod.normalize = QuantileNorm.normalize,
outputDir = file.path(results.dir, "normalization/FCS_norm"),
prefix = "Norm_",
clean = TRUE,
verbose = TRUE)
Here is an example of the files I find in the specified outputDir:
Correctly named sample example (there are 8 files, as expected):
Norm_BAS_SUR_AA22008_2022-03-14_Peanut1_QC.fcs
Unknown files example names (there are 100 of these files):
C__Users_emili_Desktop_emilier_flowSOM_results_QC_PeacoQC_results_fcs_files_BAS_SUR_AA23013_2024-07-31_Peanut_QC.fcs_fsom1.fcs
What is the potential cause for these unknown files to be created and not removed ? What should I change to the script to fix this issue?
Thank you!
The text was updated successfully, but these errors were encountered: