STOmics microscope ImageQC software is intended for assessing the quality of microscope images. Besides resolution and sharpness, it also evaluates stitching quality of a Stereo-seq chip image by referring to the background track lines. The ImageQC software has two main applications. Firstly, with the aid of a STOmics Microscope Assessment Chip T (1cm*1cm) (STOmics, Cat. No.: 101CT111), the software can be used to assess if a microscope is compatible with the STOmics protocols. Secondly, a fluorescent image of a tissue mounted Chip T (STOmics, Cat. No.: 111CT114) should pass the ImageQC evaluation to ensure that it fulfills requirements for the downstream data analysis via SAW pipelines.
• Hardware requirement: memory of at least 16G.
• Operating system: Windows 10 - 64 bit
• Connection requirement: not necessary unless image uploading to a computer cluster or cloud is required (refer to section 4.2, step 10 for details).
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