Tutorial here.
This dataset includes processed (radially averaged and mask-corrected) SAXS data from a sample of bovine serum albumin (BSA).
Data can be found in the scattering_data/processed folder.
The rest of the data (averaged, subtracted, output of various analysis softwares) is saved in the described structure below.
├── README.md <- The top-level README for developers using this project.
│
├── processed_data
│ ├── DAMMIF <- Output of DAMMIF.
| ├── GNOM <- Output of GNOM.
| └── series <- Output of the series analysis in RAW.
|
├── scattering_data
| ├── processed <- Integrated SAXS data acquired.
| ├── subtracted <- The final, buffer-subtracted averaged scattering data for modeling.
||
└── workspaces <- RAW workspaces.
Scattering data was acquired as 60 seconds exposure frames over the course of the SEC, at a flow rate of 0.1 ml/min during the elution. The sample capillary temperature was set to 20 °C and the MAXS detector configuration was used (detector distance from sample of 600 mm).
The data was acquired on 2020-01-23 by Normand Cyr at the Structural Biology Platform of the Université de Montréal.
- Instrument: Xenocs BioXolver L
- X-rays generator: Excillum MetalJet D2+ 70 kV (λ = 1.34 Å)
- Detector: Dectris PILATUS3 R 300K
- SEC system: GE Healthcare Life Sciences ÄKTAmicro coupled to a Superdex 200 10/300 Increase column
The buffer used was 20 mM Tris pH 7.5, 150 mM NaCl.