SMIS 2.3 for WINDOWS and LINUX July 11 2024

New features of SMIS 2.3

Introduce more versatility for fluorescent states that are in rapid exchange: (not all fluorescent states need anymore to be either in rapid exchange or not: some fluorescent states can be in rapid exchange and others not)

Introduce hop diffusion:

Introduce interactive drawing of FRAP zone

Added more flexible laser beam profile options, and the possibility to load experimental laser beam profiles (.tif images)

Added more flexible drift options and possibility to save drift data

Virtual sample image now part of SMIS parameters (not only path to image)

Moved new fluorophore definition menu to main SMIS GUI

Added routine to automatically update input SMIS simulation .mat files to new SMIS versions

Corrected bug in DisplayLasers when one of the laser intensity was set to 0

Corrected bug concerning the contribution of different fluorescent states with their associated pointspread function and recorded number of photons to the detected images.

Corrected but for creation of 2D and 3D NPC qPALM images in Create Virtual Samples menu

Corrected bug for placing molecules in multicolor qPALM setups

SMIS 2.3 for MACOS 24 June 2024

New features of SMIS 2.3

Introduce more versatility for fluorescent states that are in rapid exchange: (not all fluorescent states need anymore to be either in rapid exchange or not: some fluorescent states can be in rapid exchange and others not)

Introduce hop diffusion:

Introduce interactive drawing of FRAP zone

Added more flexible laser beam profile options, and the possibility to load experimental laser beam profiles (.tif images)

Added more flexible drift options

Virtual sample image now part of SMIS parameters (not only path to image)

Moved new fluorophore definition menu to main SMIS GUI

Added routine to automatically update input SMIS simulation .mat files to new SMIS versions

Corrected bug in DisplayLasers when one of the laser intensity was set to 0

Corrected bug concerning the contribution of different fluorescent states with their associated pointspread function and recorded number of photons to the detected images.

Corrected but for creation of 2D and 3D NPC qPALM images in Create Virtual Samples menu

Corrected bug for placing molecules in multicolor qPALM setups

SMIS 2.2 for WINDOWS and LINUX

=> Improve analyze results with more histograms, and corrected bug for histogram display for global on and off times
=> Introduce option for astigmatism only on channel 1 in 3D mode
=> Option to load 3D virtual sample in .tif format
=> Fixed table size for thermal exchange rate matrix and photo transformation quantum yield (for fluorophore models with a high number of states)
=> Fixed sampling rate bug associated to addtime
=> Fixed bug in Define New Fluorophore menu
=> Fixed bug in Show_Diffusion mlapp

SMIS 2.2 for MACOS

=> Improve analyze results with more histograms, and corrected bug for histogram display for global on and off times
=> Introduce option for astigmatism only on channel 1 in 3D mode
=> Option to load 3D virtual sample in .tif format
=> Fixed table size for thermal exchange rate matrix and photo transformation quantum yield (for fluorophore models with a high number of states)
=> Fixed sampling rate bug associated to addtime
=> Fixed bug in Define New Fluorophore menu
=> Fixed bug in Show_Diffusion mlapp

SMIS 2.1

Single Molecule Imaging Simulator Version 2.1