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16S rRNA gene sequencing pipeline for the V4 region using QIIME2 and DADA2

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SoliLuneStelle/16S-V4_QIIME2_DADA2

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Project Description

The files in this repository were used for the analysis of the V4 region of the 16S rRNA gene using paired-end sequencing data on the QIIME2 platform.

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Project Details

The original files for this project were downloaded from github user spacocha's PreheimLab_16S_SOPs repository and modified. The modified files are uploaded to this repository.

This work was carried out at the Advanced Research Computing at Hopkins (ARCH) core facility Rockfish, which is supported by the National Science Foundation (NSF) grant number OAC1920104, using QIIME2 version 2023.5.

QIIME2 User, Installation, & Citation Guides

For information on how to use and install QIIME2 version 2023.5 visit https://docs.qiime2.org/2023.5/.

For additional information on how QIIME2 works visit https://gregcaporaso.github.io/q2book/front-matter/reading.html.

For the Moving Pictures tutorial visit https://docs.qiime2.org/2023.5/tutorials/moving-pictures/.

For information in citing QIIME2 visit https://docs.qiime2.org/2024.2/citation/.

Contact

Please feel free to reach out with any questions at jdodd7@jhu.edu

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16S rRNA gene sequencing pipeline for the V4 region using QIIME2 and DADA2

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