Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

Sign up for GitHub

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Jump to bottom

'make_fastqs': strip trailing Ns from index sequences in sample sheets #875

Open
pjbriggs opened this issue Jul 24, 2023 · 0 comments
Open

'make_fastqs': strip trailing Ns from index sequences in sample sheets #875

pjbriggs opened this issue Jul 24, 2023 · 0 comments
Labels
enhancement Fastq generation

Comments

@pjbriggs
Copy link
Member

Proposal to strip any trailing Ns from index sequences in sample sheets in the make_fastqs command/pipeline. For example:

SM1,SM1,,,,,CCGCGGTTNNNNNNN,,CTAGCGCT,Test,

should be transformed to

SM1,SM1,,,,,CCGCGGTT,,CTAGCGCT,Test,

While BaseSpace treats Ns as matching any base, bcl2fastq cannot handle the presence of Ns.
Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Assignees
No one assigned
Labels
enhancement Fastq generation
Projects
None yet
Milestone
No milestone
Development

No branches or pull requests
1 participant
@pjbriggs