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Commands and input files for population genetic analysis of Deacon Rockfish using RADseq. Used for: Vaux F, Rasmuson LK, Kautzi LA, Rankin PS, Blume MTO, Lawrence KA, Bohn S, O’Malley KG. (2019). Sex matters: otolith shape and genomic variation in deacon rockfish (Sebastes diaconus). Ecology and Evolution 9(23), 13153-13173.

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deacon_rockfish_RADseq

Commands and input files for population genetic analysis of Deacon Rockfish using RADseq. Used for: Vaux F, Rasmuson LK, Kautzi LA, Rankin PS, Blume MTO, Lawrence KA, Bohn S, O’Malley KG. (2019). Sex matters: otolith shape and genomic variation in deacon rockfish (Sebastes diaconus). Ecology and Evolution 9, 13153-13173. https://doi.org/10.1002/ece3.5763

deacon_rockfish_STACKS_and_filtering

Lists input commands used for the STACKS analysis and subsequent analyses used for filtering in VCFtools, plink, and paralog-finder (HDplot), as well as BWA.

maps

Maps used to select individuals and populations in STACKS runs.

excluded_loci

Lists used to exclude certain loci in STACKS runs.

plink

Ped and map files used by plink to estimate loci in LD.

vcftools-hwe

Maps used to select subsets of individuals for estimating HWP.

Genetic sampling, and genotype and loci fasta files

Genetic sampling, and genetic data (vcf, genepop, and structure genotype files, and the fasta consensus file for the 92 outlier loci) are available from Dryad: https://doi.org/10.5061/dryad.8kprr4xj0

Demultiplexed reads

Demultiplexed forward and reverse DNA sequence reads for the deacon rockfish sequenced in this study are openly available on the NCBI sequence read archive (SRA) under: PRJNA560239, http://www.ncbi.nlm.nih.gov/bioproject/560239

Raw Illumina reads

Available on request from Kathleen O'Malley (State Fisheries Genomics Lab, Oregon State University), https://agsci.oregonstate.edu/state-fisheries-lab/people

Further information

See this page: https://sites.google.com/view/evauxlution/data

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Commands and input files for population genetic analysis of Deacon Rockfish using RADseq. Used for: Vaux F, Rasmuson LK, Kautzi LA, Rankin PS, Blume MTO, Lawrence KA, Bohn S, O’Malley KG. (2019). Sex matters: otolith shape and genomic variation in deacon rockfish (Sebastes diaconus). Ecology and Evolution 9(23), 13153-13173.

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