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Commands and input files for population genetic analysis of Pacific Albacore using ddRADseq. Used for: Vaux F, Bohn S, Hyde JR, O'Malley KGO. (2021). Adaptive markers distinguish North and South Pacific Albacore amid low population differentiation. Evolutionary Applications 14(5), 1343-1364.

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pacific_albacore_ddRADseq

Commands and input files for population genetic analysis of Pacific Albacore using ddRADseq

albacore_STACKS_and_filtering

Lists input commands used for STACKS and BWA, and subsequent filtering in VCFtools, plink, and paralog-finder (HDplot).

maps

Maps used to select individuals and populations in STACKS runs.

excluded_loci

Lists used to exclude certain loci in STACKS runs.

plink-ld

Ped and map files used by plink to estimate loci in LD.

vcftools-hwe

Maps used to select subsets of individuals for estimating HWP.

R

R files for running loci filtering and population genetic analyses (e.g. whoa, adegenet, fsthet). I recommmend viewing original source GitHub pages and tutorials noted in each R file credits.

Sampling, and genotype and loci fasta files

Sampling and sequence spreadsheets, genetic data (vcf, genepop, and structure genotype files, and the fasta consensus file for the 84 putatively adaptive loci), and full results for genome scan program and Fst analyses are available from Dryad: https://doi.org/10.5061/dryad.6djh9w103

Demultiplexed reads

Demultiplexed forward and reverse DNA sequence reads for the albacore sequenced in this study are openly available on the NCBI sequence read archive (SRA) under: PRJNA579774, http://www.ncbi.nlm.nih.gov/bioproject/579774

Raw Illumina reads

Available on request from Kathleen O'Malley (State Fisheries Genomics Lab, Oregon State University), https://agsci.oregonstate.edu/state-fisheries-lab/people

Further information

See this page: https://sites.google.com/view/evauxlution/data

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Commands and input files for population genetic analysis of Pacific Albacore using ddRADseq. Used for: Vaux F, Bohn S, Hyde JR, O'Malley KGO. (2021). Adaptive markers distinguish North and South Pacific Albacore amid low population differentiation. Evolutionary Applications 14(5), 1343-1364.

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