scRNA-seq Lessons from HCBC (first release)
Description
This repository has teaching materials for an Introduction to single-cell RNA-seq analysis workshop. This workshop will instruct participants on how to design a single-cell RNA-seq experiment, and how to efficiently manage and analyze the data starting from count matrices.
Learning Objectives
- Explain common considerations when designing a single-cell RNA-seq experiment
- Discuss the steps involved in taking raw single-cell RNA-sequencing data and generating a count (gene expression) matrix
- Compute and assess QC metrics at every step in the workflow
- Cluster cells based on expression data and derive the identity of the different cell types present
- Perform integration of different sample conditions