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Update README.md
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ytye2010 authored Oct 26, 2023
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10 changes: 5 additions & 5 deletions README.md
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Expand Up @@ -30,7 +30,7 @@ conda install tipars2

# Quick Usage

## Pango lineage annotation
## Ancestral lineage annotation

```bash
cd /home/ytye/tipars2_github/Benchmark_datasets/100k
Expand All @@ -52,20 +52,20 @@ cd /home/ytye/tipars2_github/Benchmark_datasets/100k

## Tree refinement
### Phylogenetic insertion using TIPars
+ include processing tree annotation
+ include processing ancestral lineage annotation
```bash
cd /home/ytye/tipars2_github/Benchmark_datasets/100k
/home/ytye/tipars2_github/tipars2 --refinement -t 100k_tree_InnodeNameAdded.nwk -s 100k_taxa.fas -a 100k_anc.fas --label 100k_pangolin.tsv --output refined_tree.nwk -T 8 -x 8G
```

+ exclude processing tree annotation that could be computed by other methods, e.g. PastML and matUtils
+ exclude processing ancestral lineage annotation that could be computed by other methods, e.g. PastML and matUtils
```bash
cd /home/ytye/tipars2_github/Benchmark_datasets/100k
/home/ytye/tipars2_github/tipars2 --refinement_from_annotation -t 100k_tree_InnodeNameAdded.nwk -s 100k_taxa.fas -a 100k_anc.fas --label 100k_pangolin.tsv --assignment 1248_in_100k_annotation.tsv --output refined_tree.nwk -T 8 -x 8G
```

### Phylogenetic insertion using UShER
+ use the [#consistent_taxa]_removedTree.nwk after tree annotation by TIPars2
+ use the [#consistent_taxa]_removedTree.nwk after ancestral lineage annotation by TIPars2
```bash
cd /home/ytye/tipars2_github/Benchmark_datasets/100k
/home/ytye/tipars2_github/tipars2 --annotation -t 100k_tree_InnodeNameAdded.nwk --label 100k_pangolin.tsv --output 1248_in_100k_annotation.tsv -T 8
Expand All @@ -80,7 +80,7 @@ usher -v taxa.vcf -t 81784_removedTree.tree -d ./usher -o ./usher/81784_AddTo_10

# Acknowledgements

This project is supported by the Hong Kong Research Grants Council General Research Fund (17150816), the NSFC Excellent Young Scientists Fund (Hong Kong and Macau) (31922087),
This project is supported by the Theme Based Research Scheme (T11-705/21-N), the Hong Kong Research Grants Council General Research Fund (17150816), the NSFC Excellent Young Scientists Fund (Hong Kong and Macau) (31922087),
the Health and Medical Research Fund (COVID1903011-549 WP1) and the Innovation and Technology Commission’s InnoHK funding (D<sup>2</sup>4H).


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