GuideScan2 is a tool for genome-wide CRISPR guide RNA (gRNA) design and analysis in custom genomes. A complete description and usage information can be found in our manual.pdf. If you found this tool useful for your research, please cite us at:
@article{schmidt2025genome,
title={Genome-wide CRISPR guide RNA design and specificity analysis with GuideScan2},
author={Schmidt, Henri and Zhang, Minsi and Chakarov, Dimitar and Bansal, Vineet and Mourelatos, Haralambos and S{\'a}nchez-Rivera, Francisco J and Lowe, Scott W and Ventura, Andrea and Leslie, Christina S and Pritykin, Yuri},
journal={Genome Biology},
volume={26},
number={1},
pages={1--25},
year={2025},
publisher={Springer}
}