Client for sh-miR Designer RESTful API
Before usage you need to set up url in settings.py!
Example usage:
Fold sequence via mfold:
./shmir_client.py mfold ACTGAUUUGAC
Create sh-miR from one siRNA strand (active) or two siRNa strands separated by space.
First strand is active, both are in 5-3 orientation
./shmir_client.py from_sirna UUUGUAUUCGCCCUAGCGC CGCUAUGGCGAAUACAAACA
Create sh-miR from transcript name.
Optional parameters
- --min_gc : Minimal "GC" content in strand -- default:
40 - --max_gc : Maximal "GC" content in strand -- default:
60 - --max_offtarget : Maximal offtarget of strand -- default:
10 - --mirna_name : The name of miRNA backbone to use -- default:
"all" - --immuno : One of
["yes", "no", "no_difference"]-- default:"no_difference"
./shmir_client.py from_transcript NM_001618.3 --mirna_name miR-30a --min_gc 22