-
Notifications
You must be signed in to change notification settings - Fork 5
3.1 ACTION
Metabolomics and DNA methylation data assessed for participants of the Netherlands Twin Register (NTR) [5] ACTION Biomarker Study (Aggression in Children: Unraveling gene-environment interplay to inform Treatment and InterventiON strategies)[6-8] were analyzed to demonstrate the pipeline from generation of ISA metadata file and importing of ISA metadata to the FDP.
ACTION is a large international consortium aimed at investigating the antecedents, characteristics, and consequences of childhood aggressive behavior (https://www.action-euproject.eu/). In the ACTION Biomarker Study, first-morning urine and buccal-cell samples were collected [9] for (epi)genomic and metabolomic measurements in twin pairs selected on aggressive behavior with the goal to identify biomarkers for childhood aggression. Details on the ACTION Biomarker Study and the omics data obtained therein have been previously published Hagenbeek et al., 2020, Front. Psychiatry,van Dongen et al., 2021, Molecular Psychiatry, Hagenbeek et al. 2022, Behav Genet, Hubers et al, 2022
We capture the experimental metadata of the ACTION multi-omics data set (DNA methylation profiles, metabolomics) using the ISA metadata framework.
We developed a Jupyter notebook script that creates both ISA-Tab files and an ISA-JSON file with the metadata mentioned above, and delivered it in a docker container.
We also developed a script for importing the Investigation file to FDP and a docker container as well.